Illustration of Glycolysis & Glycolytic Flux 

Illustration of Glycolysis and Glycolytic Flux featuring Glucose, Glycolysis, Pyruvate, 2-DG, Lactate, ATP, Mitochondria, and Oligomycin

Recent research across a diversity of fields is fuelling a renewed interest in glycolytic flux measurements, as glycolysis is highlighted again and again as a central player in disease progression and related metabolic adaptation. This is particularly true in areas such as Cancer, Hypoxia, Diabetes, Stem Cell biology and Immunometabolism. Glycolytic activity can be conveniently assessed by measuring lactate-derived acidification and, using the pH-Xtra™ Glycolysis Assay, this is now possible on conventional fluorescence plate readers. Combined with NEW pH-Xtra™ Stress Test, researchers now have the tools to conveniently and comprehensively characterise both basal glycolysis and glycolytic capacity, while avoiding the need for expensive dedicated instrumentation and complicated corrections for CO2 derived acidification.

The pH-Xtra™ Glycolysis Assay makes extracellular acidification measurements easy. A buffer tablet is provided for simple assay set-up, while the pH-Xtra™ reagent allows complete flexibility regarding the microplate used, providing a high-throughput fluorescence-based solution and facilitating measurement of both 2D and 3D models.

The pH-Xtra™ Stress Test provides the additional reagents and controls necessary to generate a complete glycolytic profile. Glucose concentration can be varied as required while the inclusion of specific metabolism modulators, including 2DG and oligomycin, enable the measurement of key metabolic parameters including glycolytic capacity; a measure of the cells ability to increase glycolytic rates upon sudden increase in demand for glycolytic ATP.

Leave a Comment