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Food Safety
Product name: Redlight
The Redlight assay provides rapid total viable counts (TVC) of food samples by measuring bacterial oxygen consumption and equating consumption rate to microbial load.
The assay is a simple 'mix and measure' test. Homogenates of food samples are prepared as for agar plates, dispensed into the wells of a standard 96-well plate together with the Redlight oxygen-sensing probe, covered with mineral oil, and measured automatically on a fluorescent reader at 30°C. The assay is based on the ability of O2 to quench the excited state of Redlight, a soluble, phosphorescent oxygen-sensitive probe.
Benefits
- The test provides sensitive, rapid enumeration of bacterial load without the need to conduct lengthy and tedious colony counts on agar plates
- Fast, simple, high throughput, assay using standard plates and fluorescent reader
- Samples prepared, and assays run, and results obtained in a single 8 hour working shift
- Data obtained and counts calculated automatically
- One 96-well microtitre plate equivalent to approx. 1000 agar plates
- Easy sample preparation, same as for agar plate. Total assay preparation time – under 1hr per plate (up to 96 samples)
- 104 Cfu/g and higher detected in hours, 108 Cfu/g and higher detected in minutes
- The software provides easy-to-read graphical representation of measured fluorescent profiles in real time, as shown by the examples in the figure below, allowing the operator to make visual semi-quantitative analysis