Our partner company Mocon now sell and market the GreenLight™ 900 series globally. For more information on the GreenLight™ 900 series, please visit www.mocon.com/foodsafety.php

GreenLight™ Model 960, is a high-throughput test that provides total aerobic viable counts (TVCs, APCs) for food samples by measuring bacterial oxygen consumption and equating oxygen consumption to microbial load. As bacteria in the test sample grow and respire they deplete O₂ which is detected as an increase in the GreenLight™ probe signal above the baseline level. The time required to reach this increase in signal is used to calculate the CFU/g of the original food sample, based on a pre-determined calibration. 

The assay is based on a 96 well plate format to facilitate a high throughput of samples. It is a simple ‘mix and measure’ protocol. Homogenates of food samples are prepared as for traditional TVC testing on agar plates (ISO:4833:2003 ref method), dispensed in the wells of a standard 96-well plate together with the GreenLight™ oxygen-sensing probe, covered with mineral oil to exclude ambient O₂ and measured on a fluorescence reader at 30^oC. Ninety-four food samples excluding controls are read simultaneously, with samples prepared and measured within hours. Total time to prepare a plate for analysis, including sample preparation is under 1 hour. 

GreenLight™ Model 960, is AOAC RI PTM certified for raw meat and poultry. The assay enables the detection and enumeration of total viable counts (TVCs, APCs) within 12 hours at contamination levels, low (10³ CFU/g) to high (10⁸ CFU/g) from the selected raw meats; ground beef, lamb, pork, chicken, turkey.

 GreenLight™ Model 960 assay is suitable for research and development studies within industrial and academic food laboratories. The test provides results within hours including sample preparation and incubation times, allowing rapid, easy and sensitive same-day enumeration of bacterial load (CFU/g) without the need to conduct multiple sample dilutions and lengthy, tedious counts on agar.

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