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MitoXpress-Intra

Plate-reader-based analysis of intracellular oxygen levels

The MitoXpress™ oxygen probe is passively loaded into cell cytosol by means of a selected loaded reagent*. After washing, the cells are monitored using a high sensitivity TR-F plate-reader using advanced lifetime measurement mode. As the test material respires, O₂ is depleted which is seen as an increase in probe phosphorescence signal. The assay is reversible, a decrease in oxygen consumption (an increase in O₂ levels) is seen as a decrease in probe signal.

Benefits

Measures rapid, transient changes in intracellular oxygen levels
The assay is easily adopted in labs, without the need for specialised equipment.

For a more detailed protocol see the application note.

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Catalogue Number	Product Description	Quantity
MitoXpress-Intra-Ki	Complete Kit: contains 1 vial of MitoXpress –3X probe and 1 vial of Endo-Porter* loading agent in DMSO solution. Loading agent also available in aqueous solution, see note below.
MitoXpress-3X	1 vial of MitoXpress-3X probe only : For use with loading agent/technique of your choice.

Note: High-throughput packages available on all products. Please contact us for more information.

* Loading Reagent: We have evaluated a range of loading techniques and reagents and found Endo-Porter to be the optimal loading agent for certain cell types, for example HepG2s. For other cell types, other loading reagents or techniques may be more appropriate. Endo-Porter contains low concentrations of DMSO that may trigger some undifferentiated cell types to differentiate. In this case, Gene Tools provide Aqueous Endo-Porter, although the aqueous formulation is considerably less effective than the DMSO formulation. Choose the loading agent or technique that works best for your cell type. For more information on Endo-Porter see www.gene-tools.com.