GreenLight™ Model 960, is a high-throughput test that provides total aerobic viable counts (TVCs, APCs) for food samples by measuring bacterial oxygen consumption and equating oxygen consumption to microbial load. As bacteria in the test sample grow and respire they deplete O₂ which is detected as an increase in the GreenLight™ probe signal above the baseline level. The time required to reach this increase in signal is used to calculate the CFU/g of the original food sample, based on a pre-determined calibration. 

 The assay is a simple ‘mix and measure’ test. Homogenates of food samples are prepared as for traditional TVC testing on agar plates (ISO:4833:2003 ref method), dispensed in the wells of a standard 96-well plate together with the GreenLight™ oxygen-sensing probe, covered with mineral oil to exclude ambient O₂ and measured on a fluorescence reader at 30^oC. Ninety-six food samples including controls (test media/buffer plus GreenLight™ probe) are read simultaneously, with samples prepared and measured within hours. Total time to prepare a plate for analysis, including sample preparation is under 1 hour. 

GreenLight™ Model 960, AOAC RI PTM certified assay enables the detection and enumeration of total viable counts (TVCs, APCs) within 12 hours at contamination levels, low (10⁴ CFU/g) to high (10⁸ CFU/g) from the selected raw meats; ground beef, lamb, pork, chicken, turkey.

Visit the Mocon booth #117 to learn more about the product.

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